Quantitative and Qualitative Studies of Chemical Transformation of Cloned C3H Mouse Embryo Cells Sensitive to Postconfluence Inhibition of Cell Division1
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چکیده
A line of C3H mouse embryo cells (C3H/10T1/2 clone 8) sensitive to postconfluence inhibition of cell division was used for a variety of quantitative and qualitative studies of chemical oncogenesis in culture. The polycyclic hydro carbons (PH), 3-methylcholanthrene, dibenz[a,h]anthracene, and 7,12-dimethylbenz[a]anthracene, caused varying degrees of cytotoxicity and produced morphologically and malignantly transformed foci in these cells with a dosedependent frequency, while yV-methyl-/V'-nitro-./V-nitroso-
منابع مشابه
Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.
SUMMARY A line of C3H mouse embryo cells (C3H/10T1/2 clone 8) sensitive to postconfluence inhibition of cell division was used for a variety of quantitative and qualitative studies of chemical oncogenesis in culture. The polycyclic hydro carbons (PH), 3-methylcholanthrene, dibenz[a,h]anthra-cene, and 7,12-dimethylbenz[a]anthracene, caused varying degrees of cytotoxicity and produced morphologic...
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We have developed a qualitative and quantitative assay system for detecting cells lacking postconfluence inhibition of cell division (contact insensitivity, CS~) in golden Syrian ham ster embryo cells in culture by measuring the number of cells able to form colonies on a lethally irradiated, confluent monolayer of a contact-sensitive established cell line. A subpopulation in normal low-passage ...
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تاریخ انتشار 2006